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Isolation, purification and separation

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Nature Protocols

Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry »

Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells »

Practical cell-free protein synthesis system using purified wheat embryos »

Regenerating cortical connections in a dish: the entorhino-hippocampal organotypic slice co-culture as tool for pharmacological screening of molecules promoting axon regeneration »

Automated solid-phase microextraction and thin-film microextraction for high-throughput analysis of biological fluids and ligand–receptor binding studies »

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Protocols Network

High-throughput homogenization of Drosophila Melanogaster larvae using a bead mill-style homogenizer. »

Rapid, high-throughput homogenization of embryonic or larval zebrafish (Danio rerio) »

FACS-GAL isolation of β-galactosidase expressing cells from mid gestation mouse embryos »

A useful procedure to isolate simultaneously DNA and RNA from a single tumor sample. »

A Protocol for FlyFos clone amplification »

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