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Immunological techniques

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Nature Protocols

Multichannel wholemount fluorescent and fluorescent/chromogenic in situ hybridization in Xenopus embryos »

Screening individual hybridomas by microengraving to discover monoclonal antibodies »

Determination of gene expression patterns using high-throughput RNA in situ hybridization to whole-mount Drosophila embryos »

Use of in vivo biotinylation to study protein–protein and protein–DNA interactions in mouse embryonic stem cells »

Labeling of biotin antibodies with horseradish peroxidase using cyanuric chloride »

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Protocols Network

mRNA expression analysis by Real-Time PCR. »

Generation of mouse bone marrow-derived dendritic cells (BM-DCs) »

Generation of mouse bone marrow-derived macrophages (BM-MFs) »

Drosophila Schneider 2 (S2) cell culture »

Real-Time calcium transient measurement in mouse dendritic cells stimulated with LPS or ATP. »

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