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Immunological techniques

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Nature Protocols

Production of glycoprotein-deleted rabies viruses for monosynaptic tracing and high-level gene expression in neurons »

Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry »

Synchronous infection of SIV and HIV in vitro for virology, immunology and vaccine-related studies »

Isolation and enrichment of embryonic mouse motoneurons from the lumbar spinal cord of individual mouse embryos »

Microwave-assisted synthesis of triple-helical, collagen-mimetic lipopeptides »

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Production of polyclonal antibodies against protein antigens purified by electroelution from SDS-polyacrylamide gel »

Co-immunoprecipitation of human mitochondrial proteases AFG3L2 and paraplegin heterologously expressed in yeast cells »

Quantifying in vivo recruitment of naïve CTL towards individual dendritic cells by standard immunofluorescence histology »

In vitro analysis of cytotoxic T cell recruitment mediated by the DC-derived chemokine CCL17 »

Preparation of peptidoglycan-depleted soluble microbial extract. »

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