This Protocol is listed in the following Categories:
Cell and tissue culture, Genetic modification, Microbiology and virology, Model organisms

Author(s): R Daniel Gietz
Affiliation(s): Department of Biochemistry and Medical Genetics, University of Manitoba
DOI: 10.1038/nprot.2007.16

Microtiter plate transformation using the LiAc/SS carrier DNA/PEG method

Here, we describe a protocol that has been adapted for the transformation of yeast cells in 96-well microtiter plates. This protocol can be tailored for multiple applications and is suitable for high-throughput applications. It can be completed in 2–3 h, once the yeast cells have been grown depending on the heat shock used.

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