This Protocol is listed in the following Categories:
Chemical modification, Pharmacology and toxicology

Author(s): Jorge O Escobedo, Weihua Wang and Robert M Strongin
Affiliation(s): Department of Chemistry, Louisiana State University
DOI: 10.1038/nprot.2006.399

Use of a commercially available reagent for the selective detection of homocysteine in plasma

A procedure for the detection of homocysteine (Hcy) in blood plasma is described. A commercially available chromogen is added to the plasma sample. The plasma solution turns from yellow to blue upon heating for 4 min when a detectable threshold level of Hcy is present. Chromatographic separations and immunogenic materials are not needed. The protocol takes ~30 min.

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